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As a result, a systematic strategy was adopted to solubilize the GST-tagged proteins and bypass the refolding step, thereby shortening the time between the growth of cells until dialysis of the purified protein

For each sorting reaction, 150 L GSH magnetic beads (70,601100, Beaverbio) were prepared by twice washing with 1 ml PBS and resuspended in 500 L binding buffer (DMEM with 2% FBS)

J Pharmacol Sci 127:1016 Herrando-Grabulosa M, Gaja-Capdevila N, Vela JM, Navarro X (2021) Sigma 1 receptor as a therapeutic target for amyotrophic lateral sclerosis

doi:10.1111/j.1468-2494.2009.00490.x

This action slows down gastric emptying, reduces appetite, and enhances feelings of satiety

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