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Oxidative stress occurs from normal cellular processes but increasingly from pollution, toxins, stress, and other exposures of modern life
For immunochemistry staining, the sections were treated with 3% hydrogen peroxide for 10 min to block endogenous peroxidase activity, and incubated in buffered normal horse serum to prevent the non-specific binding of antibodies
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Since no commercial cystine-free IMDM is available, we customized IMDM based on a DMEM medium (#D9800-28, USBiological, Salem, MA) without amino acids, glucose, sodium bicarbonate, and sodium pyruvate
As a result of the absence of corresponding histologic landmarks in the oral mucosa (ie, papillary and reticular dermis), Clark levels of cutaneous melanoma are not applicable to those of the oral cavity
Raw data from patient biopsies in Fig
