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10.1136/openhrt-2018-000993 Open Heart
The study's results suggested that the various IGF-1 analogs, including Receptor Grade IGF-1 LR3, appear to potentially reverse adverse myostatin and prevent apoptosis
PMID 1731620
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Proliferation Assay Fresh isolated PBMCs or purified T/B cells labeled carboxyfluorescein succinimidyl ester (CFSE) were cultured with different concentration of L-Cn (Sigma-Aldrich) for 5 days in the presence of soluble anti-CD3 (5 g/mL, Biolegend) and anti-CD28 (5 g/mL, Biolegend) for T cell proliferation assay or 7 days in the presence of CPG (10 g/mL, InvivoGen) for B cell proliferation assay or with medium only as control
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