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The cells were continuously superfused at a rate of 60 l/min with EC4 (ESM Table 2)

The flow through was collected next day and concentrated with a 100 kDa molecular weight cut-off concentrator

Weight loss was consistent and progressive, starting at 3.93% at three months and reaching 7.87% at twelve months. In addition, several case series studies have demonstrated benefits of GLP-1s in kidney and pancreas transplant recipients, including lowering blood glucose levels and providing renal protective effects. GLP-1 RAs would be advantageous in liver transplant recipients due to their renal clearance, avoiding interference with immunosuppressant drug metabolism

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