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Because inhomogeneous cellular distribution of reacted and unreacted probes may bias the ratiometric calculations, especially at the subcellular resolution, we opted for RT instead of RT-NH 2 to achieve high-quality quantitative results in living cells
When preparing for an assay, remove the vial, equilibrate it to ambient room temperature, and reconstitute using high-purity DMSO or preferred laboratory solvents
Quality indicators include third-party purity testing with certificates of analysis available, proper storage and shipping conditions, appropriate pricing (extremely cheap peptides often indicate quality compromises), and established reputation from trustworthy sources
It has been shown to facilitate long-chain fatty acid entry into cellular mitochondria, thereby delivering substrate for oxidation and subsequent energy production
Endocrinology 152 (9), 33623372 (2011)
Miyamoto, S
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