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HepG2 cells were resuscitated and then cultured in an incubator with 5% CO 2 at 37 C in DMEM medium (4.5 g/l glucose) supplemented with 10% fetal bovine serum (FBS) and 0.2% antibiotics, and the medium was changed every 3 days
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The rescue of cell viability by Sp was measured by following a similar treatment regimen as described above for ROS profiling of the cells
Strength: Glutathione 1000mg
Specifications Reaction volumes 5 mL (0.63.5 mL) 10 mL (3.510 mL) 30 mL (4.520 mL) Flow rate 650 mL/min Temperature range 40100 C Agitation Oscillating mixing unit Location A laboratory fume hood with the capacity to handle leakage of solvents and provide suitable airflow to prevent the build-up of flammable vapors
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