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Direct injection onto lyophilized peptide creates mechanical turbulence that can cause aggregation, denaturation of the tertiary structure, or foaming
PMID: 17004982
For a subcutaneous B12 injection, a finer, shorter needle is used: around 25 to 27 gauge and half to five-eighths of an inch
Standard reconstitution uses 2 mL of bacteriostatic water per 5 mg vial, producing a concentration of 2.5 mg per mL or 0.25 mg per 0.1 mL (10 units on an insulin syringe)
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